Improved methodology of in vivo mass multiplication of entomopathogenic nematodes: A remarkable bio agent for soil arthropod pests management

In the process of in vivo multiplication EPN, Infective Juvenile (IJs) enters into body host insect; Galleria mellonella (wax moth) larvae through natural openings and cuticle. They release symbiotic bacteria Photorhabdus luminescens insect body. The multiply hemocoel, cause septicemia disease kills within 48 hours, multiplies complete 2-3 generations inside With proven effectiveness demonstrated recent years, demand EPNs is increasing day by day. production has been mainly restricted up to laboratory level only due low recovery rats IJs existing method. Recovery EPN method labor intensive, time consuming inconvenient for mass at large scale. standardizations technology with equipment modifications have optimized small scale entrepreneurs. higher recoveries 241424 IJs/GC it as compared white trap methodology 197445 IJs/GC. This first scalable system India entomopathogenic nematode; based on LOTEK. present study we describe an improved version which bulk conditioning, harvesting, separations are automated reduce costs.